Journal: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology

Article Title: Coaggregation of FcεRI with FcγRIIB Inhibits Degranulation but Not Induction of Bcl-2 Family Members A1 and Bim in Mast Cells

doi: 10.1186/1710-1492-2-3-87

Figure Lengend Snippet: Inhibition of IgE-mediated mast-cell degranulation by FcγRIIB . A , Mast cells were sensitized with 0.1 μg/mL of monoclonal anti-dinitrophenyl (anti-DNP) clone SPE-7 immunoglobulin E (IgE) mouse antibody (anti-DNP IgE) before being challenged with 30 μg/mL of rabbit anti-mouse (RAM) F(ab') or 45 μg/mL of RAM immunoglobulin G (IgG) for 30 minutes. Resting cells or cells incubated with 0.1 μg/mL of anti-DNP IgE served as a background control. The figure represents mast-cell activation estimated by β-hexosaminidase release as measured by absorbance. B , Mast cells were sensitized with 0.1 μg/mL of monoclonal anti-DNP clone SPE-7 IgE mouse antibody (anti-DNP IgE) and preincubated with or without 5 μg/mL of RAM CD16/CD32 (FcγIII/II receptor) monoclonal antibody (2.4G2 rat Ab), before challenge with 10 μg/mL of TNP-F(ab') mouse anti-rat (MAR) for 30 minutes. Cells incubated with 0.1 μg/mL of anti-DNP IgE or 0.1 μg/mL of anti-DNP IgE and 5 μg/mL of 2.4G2 rat Ab served as a background control ( n = 6; results are presented as mean ± standard error of the mean). * p < .05.

Article Snippet: Purified RAM CD16/CD32 (FcγIII/II receptor) monoclonal antibody (2.4G2 rat Ab) was obtained from BD Biosciences, Heidelberg, Germany.

Techniques: Inhibition, Incubation, Activation Assay

Journal: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology

Article Title: Coaggregation of FcεRI with FcγRIIB Inhibits Degranulation but Not Induction of Bcl-2 Family Members A1 and Bim in Mast Cells

doi: 10.1186/1710-1492-2-3-87

Figure Lengend Snippet: Reduction of immunoglobulin E (IgE)-dependent phosphorylation of Akt by FcγRIIB . C57 mast cells were activated as in Figure 1B for the indicated periods of time. Cell lysates were prepared, and the phosphorylation of Akt was analyzed by Western blot with the indicated antibodies. The result is representative of three independent experiments. Ser = serine; Thr = threonine; 2.4G2 Ab = anti-mouse CD16/CD32 (FcγIII/II receptor) monoclonal antibody.

Article Snippet: Purified RAM CD16/CD32 (FcγIII/II receptor) monoclonal antibody (2.4G2 rat Ab) was obtained from BD Biosciences, Heidelberg, Germany.

Techniques: Western Blot

Journal: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology

Article Title: Coaggregation of FcεRI with FcγRIIB Inhibits Degranulation but Not Induction of Bcl-2 Family Members A1 and Bim in Mast Cells

doi: 10.1186/1710-1492-2-3-87

Figure Lengend Snippet: Phosphorylation of Foxo3a induced by FcεRI and FcγRIIB or by FcεRI alone . C57 mast cells were activated as in Figure 1B for the indicated periods of time. Cell lysates were prepared, and the phosphorylation of Foxo3a was analyzed by Western blot with the indicated antibodies. The result is representative of three independent experiments. Ser = serine; Thr = threonine; 2.4G2 Ab = anti-mouse CD16/CD32 (FcγIII/II receptor) monoclonal antibody.

Article Snippet: Purified RAM CD16/CD32 (FcγIII/II receptor) monoclonal antibody (2.4G2 rat Ab) was obtained from BD Biosciences, Heidelberg, Germany.

Techniques: Western Blot

Journal: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology

Article Title: Coaggregation of FcεRI with FcγRIIB Inhibits Degranulation but Not Induction of Bcl-2 Family Members A1 and Bim in Mast Cells

doi: 10.1186/1710-1492-2-3-87

Figure Lengend Snippet: Expression of Bim induced by FcεRI and FcγRIIB or by FcεRI alone . C57 mast cells were activated as in Figure 1B for 24 hours. Cells sensitized only with 0.1 μg/mL of anti-dinitrophenyl immunoglobulin E (lgE) and incubated with or without 5 μg/mL of rabbit anti-mouse CD16/CD32 (FcγIII/II receptor) monoclonal antibody (2.4G2 rat Ab) were used as controls. Cell lysates were prepared, and the induction of Bim was analyzed by Western blot with the indicated antibodies. The result is representative of three independent experiments. MAR = mouse anti-rat; TNP = trinitrophenyl.

Article Snippet: Purified RAM CD16/CD32 (FcγIII/II receptor) monoclonal antibody (2.4G2 rat Ab) was obtained from BD Biosciences, Heidelberg, Germany.

Techniques: Expressing, Incubation, Western Blot